This method is based on microparticle bombardment followed by selection of transformants using the antibiotic nourseothricin. Diatoms are unicellular algae with plastids acquired by secondary endosymbiosis. We report the 34 million–base pair draft nuclear genome of the marine diatom Thalassiosira pseudonana and its 129 thousand–base pair plastid and 44 thousand–base pair mitochondrial genomes. This genome instability is potentially due to the lower G+C DNA content of T. pseudonana (30%) compared to P. tricornutum (35%). A hallmark of diatoms is their intricately patterned silicified cell wall. The genome of the diatom Thalassiosira pseudonana: ecology, evolution, and metabolism. In contrast to higher plants, diatoms acquired their plastids via secondary endosymbiosis, the merging of a eukaryotic host cell with a eukaryotic unicellular photosynthetic alga. Thalassiosira pseudonana is a species of marine centric diatoms.It was chosen as the first eukaryotic marine phytoplankton for whole genome sequencing. They are responsible for ∼20% of global carbon fixation. Please use both portals to make a complete analysis of the genome. Thalassiosira pseudonana is a marine diatom, a unicellular photosynthetic alga. Here, we describe the first system for genetic transformation of Thalassiosira pseudonana (Hustedt) Hasle et Heimdal, the only diatom for which a complete genome sequence is presently available. These include genome sequences for a few free-living marine protists such as the red algae Cyanidioschyzon merolae, the diatom Thalassiosira pseudonana, the prasinophytes O. tauri and Ostreococcus lucimarinus, the green algae Chlamydomonas reinhardtii, and the ciliates Paramecium tetraurelia and Tetrahymena thermophila. T. pseudonana was selected for this study because it is a model for diatom physiology studies, belongs to a genus widely distributed throughout the world's oceans, and has a relatively small genome at 34 mega base pairs. Using the model species Thalassiosira pseudonana , we conducted a detailed physiological and transcriptomic survey to measure the recurrent transcriptional changes that characterize typical … Genome analysis reveals that T. pseudonana contains three silicon transporters and three silaffin genes . P. tricornutum , however, is an atypical diatom in that it does not have an obligate requirement of silicon for growth and exists as three distinct morphotypes: oval, … We report the 34 million–base pair draft nuclear genome of the marine diatom Thalassiosira pseudonana and its 129 thousand–base pair plastid and 44 thousand–base pair mitochondrial genomes. Genome Insider Episode 7: Decoding Yellowstone’s microbial mats In Hot Spring Microbial Mat, Viruses Ride “Piggyback” The More the Merrier: Making the Case for Plant Pan-genomes Effects of Polar Light Cycle on Microbial Food Web Genome Insider (Mini-)Episode … To better understand the interplay between redox balance and organic matter secretion, we reconstructed a genome-scale metabolic model of Thalassiosira pseudonana strain CCMP 1335, a model for diatom molecular biology and physiology, with a 60-year history of studies. The marine centric diatom Thalassiosira pseudonana was chosen as the first eukaryotic marine phytoplankton for whole genome sequencing because this species has served as a model for diatom physiology studies, the genus Thalassiosira is cosmopolitan throughout the world's oceans, and the genome is relatively small at 34 mega base pairs. They do so, in theory, by sensing changing conditions and adapting their physiology accordingly. PLEASE NOTE: The Thalassiosira pseudonana genome sequence is composed of "finished chromosomes" (Thaps3) and "unmapped sequence" (Thaps3_bd), which were annotated separately. Specifically, after approximately 60 generations of propagation in E. coli, 17% of cloned T. pseudonana mitochondrial genomes contained deletions compared to 0% of previously cloned P. tricornutum mitochondrial genomes. Data Release Policy As a public service, the raw sequence data from the Thalassiosira pseudonana genome sequencing project is … Marine diatoms are important primary producers that thrive in diverse and dynamic environments.